Hotspot

ndhH

SSC · 119202-120383 · 1182 bp

Back to Leonurus

Pi 0.00226

MarkerSeek score 60.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 4
pi: 0.00226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AGCATCCATATGATCCCATAGAC	TGAAGCGTGAGACCATTTGA	43.5	45.0	57.4	57.5	1370 bp	1.0	46.9
ndhH_p2	GCATCCATATGATCCCATAGACT	TGAAGCGTGAGACCATTTGA	43.5	45.0	57.4	57.5	1369 bp	1.0	46.9
ndhH_p3	AGCATCCATATGATCCCATAGAC	GCGTGAGACCATTTGAGCTT	43.5	50.0	57.4	58.8	1366 bp	1.0	44.5
ndhH_p4	GCATCCATATGATCCCATAGACT	AAGCGTGAGACCATTTGAGC	43.5	50.0	57.4	58.8	1367 bp	1.0	44.5
ndhH_p5	GCATCCATATGATCCCATAGACT	GCGTGAGACCATTTGAGCTT	43.5	50.0	57.4	58.8	1365 bp	1.0	44.5