Hotspot

matK

LSC · 1910-3445 · 1536 bp

Back to Leonurus

Pi 0.00174

MarkerSeek score 60.10

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1536
variable_sites: 4
pi: 0.00174
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	GGATCTTTTTATCTCTCGATCCA	AGTTTATCTGTCCCCGAGGT	39.1	50.0	55.6	57.8	1706-1712 bp	1.0	40.5
matK_p2	TGGATCTTTTTATCTCTCGATCC	AGTTTATCTGTCCCCGAGGT	39.1	50.0	55.6	57.8	1707-1713 bp	1.0	40.5
matK_p3	ACATCAACATTTCAGAATAGTGGA	AGTTTATCTGTCCCCGAGGT	33.3	50.0	56.4	57.8	1740-1746 bp	1.0	40.5
matK_p4	TTGGATCTTTTTATCTCTCGATCC	AGTTTATCTGTCCCCGAGGT	37.5	50.0	56.3	57.8	1708-1714 bp	1.0	40.5
matK_p5	GGATCTTTTTATCTCTCGATCCAT	AGTTTATCTGTCCCCGAGGT	37.5	50.0	56.1	57.8	1706-1712 bp	1.0	40.5