Hotspot

petD-rps11

LSC · 76036-77288 · 1253 bp

Back to Leonurus

Pi 0.0016

MarkerSeek score 59.80

Primer available yes

Label petD-rps11

Feature Metadata

label_name: petD-rps11
feature_type: igs
region: LSC
length_bp: 1253
variable_sites: 3
pi: 0.0016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rps11_p1	TTGGTTGGGTATTGGTGCAA	AACCCCCATGCCACATAATG	45.0	50.0	57.9	58.2	1366 bp	1.0	77.8
petD-rps11_p2	TTTGGTTGGGTATTGGTGCA	AACCCCCATGCCACATAATG	45.0	50.0	57.9	58.2	1367 bp	1.0	77.8
petD-rps11_p3	GTCGCTCTTTGGTTGGGTAT	AACCCCCATGCCACATAATG	50.0	50.0	57.9	58.2	1374 bp	1.0	77.6
petD-rps11_p4	CAGTAGCGACAACCGTCTTT	AACCCCCATGCCACATAATG	50.0	50.0	58.2	58.2	1409 bp	1.0	76.7
petD-rps11_p5	TTGGTTGGGTATTGGTGCAA	GTAACCCCCATGCCACATAA	45.0	50.0	57.9	57.6	1368 bp	1.0	75.9