Hotspot

psaJ-rpl33

LSC · 65766-66225 · 460 bp

Back to Leonurus

Pi 0.00145

MarkerSeek score 45.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 460
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGATGCGTTGACATTTCCCT	GGAAATGCCCGTTGATACCT	50.0	50.0	58.5	57.9	587-588 bp	1.0	75.6
psaJ-rpl33_p2	GGATGCGTTGACATTTCCCT	TCCAAAATCACCGTTACCCG	50.0	50.0	58.5	58.2	535-536 bp	1.0	74.5
psaJ-rpl33_p3	GGATGCGTTGACATTTCCCT	TGGAAATGCCCGTTGATACC	50.0	50.0	58.5	58.2	588-589 bp	1.0	74.0
psaJ-rpl33_p4	GGATGCGTTGACATTTCCCT	CGCATCTTTCCTCTTAGCCA	50.0	50.0	58.5	57.7	515-516 bp	1.0	73.5
psaJ-rpl33_p5	GGATGCGTTGACATTTCCCT	ATCTGGAAATGCCCGTTGAT	50.0	45.0	58.5	57.6	591-592 bp	1.0	72.4