Hotspot

rbcL-accD

LSC · 55421-56109 · 689 bp

Back to Leonurus

Pi 0.00677

MarkerSeek score 63.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 7
pi: 0.00677
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGCAAGTGGAGTCCTGAAC	CGGATCTTCGCTTTCACTAGT	50.0	47.6	58.0	57.8	912 bp	1.0	71.1
rbcL-accD_p2	TTGCAAGTGGAGTCCTGAAC	CCATAGGACCAAGATTGTCCG	50.0	52.4	58.0	58.2	886 bp	1.0	71.1
rbcL-accD_p3	CCGCTTGTGAGGTATGGAAA	CGGATCTTCGCTTTCACTAGT	50.0	47.6	57.9	57.8	886 bp	1.0	70.6
rbcL-accD_p4	CCGCTTGTGAGGTATGGAAA	CCATAGGACCAAGATTGTCCG	50.0	52.4	57.9	58.2	860 bp	1.0	70.5
rbcL-accD_p5	CTTGCAAGTGGAGTCCTGAA	CGGATCTTCGCTTTCACTAGT	50.0	47.6	57.7	57.8	913 bp	1.0	69.3