Hotspot

atpB-rbcL

LSC · 53172-53974 · 803 bp

Back to Leonurus

Pi 0.00249

MarkerSeek score 60.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 3
pi: 0.00249
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GATGATACGCCCCTGGTTTT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	925-926 bp	1.0	77.5
atpB-rbcL_p2	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	963-964 bp	1.0	76.7
atpB-rbcL_p3	ATTTGGATGATACGCCCCTG	AACACCCGCTTTGAATCCAA	50.0	45.0	57.7	58.3	930-931 bp	1.0	75.7
atpB-rbcL_p4	GATGATACGCCCCTGGTTTT	TAACACCCGCTTTGAATCCA	50.0	45.0	57.9	57.4	926-927 bp	1.0	75.4
atpB-rbcL_p5	GGCGGAAAGGCTACATCTAG	TAACACCCGCTTTGAATCCA	55.0	45.0	57.8	57.4	964-965 bp	1.0	74.5