Hotspot

atpH-atpI

LSC · 13556-14359 · 804 bp

Back to Leonurus

Pi 0.00249

MarkerSeek score 60.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 3
pi: 0.00249
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	936-941 bp	1.0	79.1
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	57.9	57.9	952-957 bp	1.0	78.2
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.2	57.9	937-942 bp	1.0	77.9
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.8	57.9	951-956 bp	1.0	77.7
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	CCCGTCATGTTCCTTGGATT	50.0	50.0	58.3	57.9	946-951 bp	1.0	77.3