Hotspot

ycf1

SSC · 107640-112757 · 5118 bp

Back to Leontopodium

Pi 0.0012

MarkerSeek score 37.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5118
variable_sites: 45
pi: 0.0012
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATCGCCTCATGTCGAATCC	TTGTTTTGAATTTGATGGACT	50.0	28.6	58.1	52.1	210 bp	1.0	41.1
ycf1_p2	AATCGCCTCATGTCGAATCC	ATTGTTTTGAATTTGATGGACT	50.0	27.3	58.1	52.8	211 bp	1.0	41.1
ycf1_p3	AATCGCCTCATGTCGAATCC	TGTTTTGAATTTGATGGACTAA	50.0	27.3	58.1	52.2	209 bp	1.0	41.1
ycf1_p4	AATCGCCTCATGTCGAATCC	TTGTTTTGAATTTGATGGACTA	50.0	27.3	58.1	52.2	210 bp	1.0	41.1
ycf1_p5	AATCGCCTCATGTCGAATCC	TGTTTTGAATTTGATGGACTAAA	50.0	26.1	58.1	53.1	209 bp	1.0	41.1