Hotspot

rpl16

LSC · 80462-81843 · 1382 bp

Back to Leontopodium

Pi 0.000478

MarkerSeek score 47.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1382
variable_sites: 8
pi: 0.000478
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2433-2444 bp	1.0	79.8
rpl16_p2	TCGACGATTGCTAGCTCCTA	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2223-2234 bp	1.0	79.7
rpl16_p3	GCGTCTTTGATCACAGCAAC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2267-2278 bp	1.0	79.7
rpl16_p4	CGCACCAAAAACTCGAGTTC	GCAAATTGCAGGACGTATCG	50.0	50.0	58.0	57.9	2116-2127 bp	1.0	79.6
rpl16_p5	ACACATCAATTCTCGAGCCC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2193-2204 bp	0.962963	78.2