Hotspot

ndhD

SSC · 119327-120895 · 1569 bp

Back to Leontopodium

Pi 0.00135

MarkerSeek score 42.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1569
variable_sites: 6
pi: 0.00135
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTATGGCATGAAACAACGCG	TTTCATTAGTCAACCACTCTT	45.0	33.3	57.7	52.7	1715-1717 bp	0.962963	38.8
ndhD_p2	TTATGGCATGAAACAACGCG	TTTTCATTAGTCAACCACTCT	45.0	33.3	57.7	52.7	1716-1718 bp	0.962963	38.8
ndhD_p3	TTATGGCATGAAACAACGCG	AGTCAACCACTCTTAAAAATGA	45.0	31.8	57.7	53.6	1708-1710 bp	0.962963	38.8
ndhD_p4	TTATGGCATGAAACAACGCG	TTTTCATTAGTCAACCACTCTT	45.0	31.8	57.7	53.6	1716-1718 bp	0.962963	38.8
ndhD_p5	TTATGGCATGAAACAACGCG	TTTTTCATTAGTCAACCACTCT	45.0	31.8	57.7	53.6	1717-1719 bp	0.962963	38.8