Hotspot

rpl32-ndhF

SSC · 123008-124024 · 1017 bp

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Pi 0.00215

MarkerSeek score 55.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1017
variable_sites: 15
pi: 0.00215
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACAAAGCTTTTAACGCTGCC	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	921-1212 bp	1.0	83.7
rpl32-ndhF_p2	AACGCTGCCCAATATCCTTT	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	910-1201 bp	1.0	83.7
rpl32-ndhF_p3	ACAAAGCTTTTAACGCTGCC	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	919-1210 bp	1.0	83.6
rpl32-ndhF_p4	ACAAAGCTTTTAACGCTGCC	TTGCCGTCGGAAAAAGAAGA	45.0	45.0	57.9	58.0	920-1211 bp	1.0	83.6
rpl32-ndhF_p5	AACGCTGCCCAATATCCTTT	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	908-1199 bp	1.0	83.6