Hotspot

trnL-UAG-rpl32

SSC · 122296-122842 · 547 bp

Back to Leontopodium

Pi 0.00603

MarkerSeek score 55.30

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 547
variable_sites: 15
pi: 0.00603
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GGCAGCGTTAAAAGCTTTGT	50.0	45.0	58.0	57.9	663-682 bp	1.0	81.4
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	GGCAGCGTTAAAAGCTTTGT	50.0	45.0	58.0	57.9	664-683 bp	1.0	81.4
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	AAAGGATATTGGGCAGCGTT	50.0	45.0	58.0	57.9	674-693 bp	1.0	81.3
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	AAAGGATATTGGGCAGCGTT	50.0	45.0	58.0	57.9	675-694 bp	1.0	81.3
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	GGCAGCGTTAAAAGCTTTGT	50.0	45.0	57.9	57.9	673-692 bp	1.0	80.4