Hotspot

accD-psaI

LSC · 58047-58895 · 849 bp

Back to Leontopodium

Pi 0.00105

MarkerSeek score 48.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 12
pi: 0.00105
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	1011-1027 bp	1.0	66.2
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	1010-1026 bp	1.0	66.2
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	TTGCGATTGCCGGAAATACT	45.0	45.0	56.2	58.3	1012-1028 bp	1.0	66.2
accD-psaI_p4	TCCAGAAGGGCTTATTCGATC	AGGCCTACTAAAGGGACCAA	47.6	50.0	57.2	57.6	991-1007 bp	1.0	65.5
accD-psaI_p5	CCAGAAGGGCTTATTCGATCT	AGGCCTACTAAAGGGACCAA	47.6	50.0	57.2	57.6	990-1006 bp	1.0	65.4