Hotspot

ndhC-trnV-UAC

LSC · 49666-50854 · 1189 bp

Back to Leontopodium

Pi 0.000906
MarkerSeek score 47.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1189
variable_sites
9
pi
0.000906
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTTGCTACTCGGAGCTA GAGAAGGTCTACGGTTCGAG 50.0 55.0 57.9 57.2 1334-1343 bp 1.0 73.0
ndhC-trnV-UAC_p2 TCCTTTGCTACTCGGAGCTA CGAGAAGGTCTACGGTTCGA 50.0 55.0 57.9 58.9 1335-1344 bp 1.0 72.3
ndhC-trnV-UAC_p3 ACTCGGAGCTAAAAATCCCG GAGAAGGTCTACGGTTCGAG 50.0 55.0 57.7 57.2 1325-1334 bp 1.0 71.7
ndhC-trnV-UAC_p4 TTCTCCGGTCCTTTGCTACT GAGAAGGTCTACGGTTCGAG 50.0 55.0 58.4 57.2 1342-1351 bp 1.0 71.3
ndhC-trnV-UAC_p5 ACTCGGAGCTAAAAATCCCG CGAGAAGGTCTACGGTTCGA 50.0 55.0 57.7 58.9 1326-1335 bp 1.0 70.9