primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4372	5154	978-984	1.000000	77.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4372	5154	977-983	1.000000	77.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4372	5154	917-923	1.000000	77.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4372	5154	916-922	1.000000	77.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4372	5154	1002-1008	1.000000	76.5
atpI-atpH_p1	atpI-atpH	1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	1.4	0.297359	24573	25680	1240-1252	0.962963	77.6
atpI-atpH_p2	atpI-atpH	2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.371251	24573	25680	1296-1308	0.962963	76.9
atpI-atpH_p3	atpI-atpH	3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.407418	24573	25680	1256-1268	0.962963	76.7
atpI-atpH_p4	atpI-atpH	4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	58.3	58.2	0.0	1.4	0.442898	24573	25680	1241-1253	0.962963	76.4
atpI-atpH_p5	atpI-atpH	5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.449288	24573	25680	1300-1312	0.962963	76.3
trnG-UCC-psbD_p1	trnG-UCC-psbD	1	AATCAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	20	20	45.0	55.0	57.0	58.0	0.0	5.0	0.975609	30360	31992	1769-1796	1.000000	74.4
trnG-UCC-psbD_p2	trnG-UCC-psbD	2	ATCAAACCGAAAGACCCCTT	GAAACGGTCCCTACGTAACC	20	20	45.0	55.0	57.0	58.0	0.0	5.0	0.975609	30360	31992	1768-1795	1.000000	74.4
trnG-UCC-psbD_p3	trnG-UCC-psbD	3	GAATCAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	21	20	47.6	55.0	58.2	58.0	0.0	5.0	1.212053	30360	31992	1770-1797	1.000000	72.5
trnG-UCC-psbD_p4	trnG-UCC-psbD	4	AATCAAACCGAAAGACCCCT	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	57.0	58.2	0.0	0.0	1.217970	30360	31992	1777-1804	1.000000	72.5
trnG-UCC-psbD_p5	trnG-UCC-psbD	5	ATCAAACCGAAAGACCCCTT	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	57.0	58.2	0.0	0.0	1.217970	30360	31992	1776-1803	1.000000	72.5
trnS-UGA-psbZ_p1	trnS-UGA-psbZ	1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.226377	34742	35078	455-459	1.000000	79.4
trnS-UGA-psbZ_p2	trnS-UGA-psbZ	2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	20	20	55.0	55.0	58.0	58.3	0.0	0.0	0.277359	34742	35078	457-461	1.000000	79.0
trnS-UGA-psbZ_p3	trnS-UGA-psbZ	3	TCAACCACTCAGCCATCTCT	CCAACCATCAGGAGAAGCAA	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.555701	34742	35078	456-460	1.000000	76.8
trnS-UGA-psbZ_p4	trnS-UGA-psbZ	4	TCAACCACTCAGCCATCTCT	GACCAACCATCAGGAGAAGC	20	20	50.0	55.0	58.4	58.3	0.0	0.0	0.606683	34742	35078	458-462	1.000000	76.4
trnS-UGA-psbZ_p5	trnS-UGA-psbZ	5	TTCAACCACTCAGCCATCTC	CCAACCATCAGGAGAAGCAA	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.684588	34742	35078	457-461	1.000000	75.7
psaA-ycf3_p1	psaA-ycf3	1	AGAAATGACCCGGTTTAGCC	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.385211	41037	41800	953-957	1.000000	78.0
psaA-ycf3_p2	psaA-ycf3	2	AGAAATGACCCGGTTTAGCC	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.386439	41037	41800	955-959	1.000000	78.0
psaA-ycf3_p3	psaA-ycf3	3	CCCATGCCTCGAATGAAGTT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.505754	41037	41800	935-939	1.000000	77.1
psaA-ycf3_p4	psaA-ycf3	4	CCCATGCCTCGAATGAAGTT	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.506982	41037	41800	937-941	1.000000	77.1
psaA-ycf3_p5	psaA-ycf3	5	GTTTAGCCCATGCCTCGAAT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.3	58.3	0.0	0.0	0.580596	41037	41800	941-945	1.000000	76.5
ycf3_p1	ycf3	1	TGATGGGGCCAACAAACAAT	TCCCGCGAGAAGTAATGAGA	20	20	45.0	50.0	58.3	58.2	4.4	8.2	0.514002	41801	43740	2098-2106	1.000000	76.5
ycf3_p2	ycf3	2	TGATGGGGCCAACAAACAAT	GGATCATTTTCCCGCGAGAA	20	20	45.0	50.0	58.3	58.3	4.4	8.2	0.609243	41801	43740	2107-2115	1.000000	75.8
ycf3_p3	ycf3	3	TGATGGGGCCAACAAACAAT	ATCATTTTCCCGCGAGAAGT	20	20	45.0	45.0	58.3	57.6	4.4	8.2	0.676190	41801	43740	2105-2113	1.000000	75.2
ycf3_p4	ycf3	4	TGATGGGGCCAACAAACAAT	CCCGCGAGAAGTAATGAGAA	20	20	45.0	50.0	58.3	57.4	4.4	8.2	0.852144	41801	43740	2097-2105	1.000000	73.8
ycf3_p5	ycf3	5	TGATGGGGCCAACAAACAAT	TTCCCGCGAGAAGTAATGAG	20	20	45.0	50.0	58.3	57.4	4.4	8.2	0.852144	41801	43740	2099-2107	1.000000	73.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46073	46620	607-615	1.000000	78.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46073	46620	639-647	1.000000	77.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46073	46620	601-609	1.000000	77.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	46073	46620	633-641	1.000000	76.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	20	20	55.0	55.0	57.9	58.6	0.0	10.2	0.749620	46073	46620	606-614	1.000000	75.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTTGCTACTCGGAGCTA	GAGAAGGTCTACGGTTCGAG	20	20	50.0	55.0	57.9	57.2	4.6	0.0	0.968198	49666	50854	1334-1343	1.000000	73.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCCTTTGCTACTCGGAGCTA	CGAGAAGGTCTACGGTTCGA	20	20	50.0	55.0	57.9	58.9	4.6	0.0	1.057432	49666	50854	1335-1344	1.000000	72.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ACTCGGAGCTAAAAATCCCG	GAGAAGGTCTACGGTTCGAG	20	20	50.0	55.0	57.7	57.2	4.6	0.0	1.139998	49666	50854	1325-1334	1.000000	71.7
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTCTCCGGTCCTTTGCTACT	GAGAAGGTCTACGGTTCGAG	20	20	50.0	55.0	58.4	57.2	0.0	0.0	1.187958	49666	50854	1342-1351	1.000000	71.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	ACTCGGAGCTAAAAATCCCG	CGAGAAGGTCTACGGTTCGA	20	20	50.0	55.0	57.7	58.9	4.6	0.0	1.229233	49666	50854	1326-1335	1.000000	70.9
rbcL_p1	rbcL	1	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTCATTGAAA	20	20	50.0	45.0	57.1	58.0	0.0	7.7	0.952221	54584	56041	1518-1521	1.000000	73.2
rbcL_p2	rbcL	2	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTCATTGAA	20	20	50.0	45.0	57.1	58.0	0.0	0.0	0.952221	54584	56041	1519-1522	1.000000	73.2
rbcL_p3	rbcL	3	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTCATTGAAA	20	20	45.0	45.0	57.0	58.0	0.0	7.7	0.973233	54584	56041	1566-1569	1.000000	73.0
rbcL_p4	rbcL	4	ACGCCTAATTCATGTCGAGT	TTGGGCCGAGTTTCATTGAA	20	20	45.0	45.0	57.0	58.0	0.0	0.0	0.973233	54584	56041	1567-1570	1.000000	73.0
rbcL_p5	rbcL	5	TCATGAGTTGTAGGGAGGGA	ATTGGGCCGAGTTTCATTGA	20	20	50.0	45.0	57.1	57.8	0.0	0.0	1.154890	54584	56041	1520-1523	1.000000	71.5
accD-psaI_p1	accD-psaI	1	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	21	20	47.6	45.0	57.2	58.3	0.0	8.0	2.008062	58047	58895	1011-1027	1.000000	66.2
accD-psaI_p2	accD-psaI	2	CCAGAAGGGCTTATTCGATCT	TTGCGATTGCCGGAAATACT	21	20	47.6	45.0	57.2	58.3	0.0	8.0	2.015348	58047	58895	1010-1026	1.000000	66.2
accD-psaI_p3	accD-psaI	3	TTCCAGAAGGGCTTATTCGA	TTGCGATTGCCGGAAATACT	20	20	45.0	45.0	56.2	58.3	0.0	8.0	2.015357	58047	58895	1012-1028	1.000000	66.2
accD-psaI_p4	accD-psaI	4	TCCAGAAGGGCTTATTCGATC	AGGCCTACTAAAGGGACCAA	21	20	47.6	50.0	57.2	57.6	0.0	1.3	2.107555	58047	58895	991-1007	1.000000	65.5
accD-psaI_p5	accD-psaI	5	CCAGAAGGGCTTATTCGATCT	AGGCCTACTAAAGGGACCAA	21	20	47.6	50.0	57.2	57.6	0.0	1.3	2.114841	58047	58895	990-1006	1.000000	65.4
ycf4-cemA_p1	ycf4-cemA	1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.106483	59973	60453	639-661	1.000000	82.1
ycf4-cemA_p2	ycf4-cemA	2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	59973	60453	571-593	1.000000	81.9
ycf4-cemA_p3	ycf4-cemA	3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.172357	59973	60453	603-625	1.000000	81.7
ycf4-cemA_p4	ycf4-cemA	4	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	59973	60453	585-607	1.000000	81.2
ycf4-cemA_p5	ycf4-cemA	5	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.297297	59973	60453	583-605	1.000000	80.8
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	62327	63090	901-908	1.000000	77.7
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	62327	63090	891-898	1.000000	77.7
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	62327	63090	939-946	1.000000	76.0
petA-psbJ_p4	petA-psbJ	4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	62327	63090	938-945	1.000000	76.0
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	62327	63090	929-936	1.000000	76.0
rpl16_p1	rpl16	1	CGCACCAAAAACTCGAGTTC	GTCGAGGGAATTGCACGTAT	20	20	50.0	50.0	58.0	58.1	29.8	13.2	0.082785	80462	81843	2433-2444	1.000000	79.8
rpl16_p2	rpl16	2	TCGACGATTGCTAGCTCCTA	GTCGAGGGAATTGCACGTAT	20	20	50.0	50.0	58.0	58.1	17.2	13.2	0.100895	80462	81843	2223-2234	1.000000	79.7
rpl16_p3	rpl16	3	GCGTCTTTGATCACAGCAAC	GTCGAGGGAATTGCACGTAT	20	20	50.0	50.0	58.0	58.1	3.9	13.2	0.108306	80462	81843	2267-2278	1.000000	79.7
rpl16_p4	rpl16	4	CGCACCAAAAACTCGAGTTC	GCAAATTGCAGGACGTATCG	20	20	50.0	50.0	58.0	57.9	29.8	13.7	0.109869	80462	81843	2116-2127	1.000000	79.6
rpl16_p5	rpl16	5	ACACATCAATTCTCGAGCCC	GTCGAGGGAATTGCACGTAT	20	20	50.0	50.0	58.0	58.1	7.8	13.2	0.093906	80462	81843	2193-2204	0.962963	78.2
ycf1_p1	ycf1	1	AATCGCCTCATGTCGAATCC	TTGTTTTGAATTTGATGGACT	20	21	50.0	28.6	58.1	52.1	10.3	0.0	7.041266	111804	111923	210-210	1.000000	41.1
ycf1_p2	ycf1	2	AATCGCCTCATGTCGAATCC	ATTGTTTTGAATTTGATGGACT	20	22	50.0	27.3	58.1	52.8	10.3	0.0	7.346232	111804	111923	211-211	1.000000	41.1
ycf1_p3	ycf1	3	AATCGCCTCATGTCGAATCC	TGTTTTGAATTTGATGGACTAA	20	22	50.0	27.3	58.1	52.2	10.3	0.0	7.929949	111804	111923	209-209	1.000000	41.1
ycf1_p4	ycf1	4	AATCGCCTCATGTCGAATCC	TTGTTTTGAATTTGATGGACTA	20	22	50.0	27.3	58.1	52.2	10.3	0.0	7.929949	111804	111923	210-210	1.000000	41.1
ycf1_p5	ycf1	5	AATCGCCTCATGTCGAATCC	TGTTTTGAATTTGATGGACTAAA	20	23	50.0	26.1	58.1	53.1	10.3	0.0	8.053133	111804	111923	209-209	1.000000	41.1
ndhD_p1	ndhD	1	TTATGGCATGAAACAACGCG	TTTCATTAGTCAACCACTCTT	20	21	45.0	33.3	57.7	52.7	11.0	0.0	6.571097	119327	120895	1715-1717	0.962963	38.8
ndhD_p2	ndhD	2	TTATGGCATGAAACAACGCG	TTTTCATTAGTCAACCACTCT	20	21	45.0	33.3	57.7	52.7	11.0	0.0	6.571097	119327	120895	1716-1718	0.962963	38.8
ndhD_p3	ndhD	3	TTATGGCATGAAACAACGCG	AGTCAACCACTCTTAAAAATGA	20	22	45.0	31.8	57.7	53.6	11.0	0.0	6.674841	119327	120895	1708-1710	0.962963	38.8
ndhD_p4	ndhD	4	TTATGGCATGAAACAACGCG	TTTTCATTAGTCAACCACTCTT	20	22	45.0	31.8	57.7	53.6	11.0	0.0	6.674841	119327	120895	1716-1718	0.962963	38.8
ndhD_p5	ndhD	5	TTATGGCATGAAACAACGCG	TTTTTCATTAGTCAACCACTCT	20	22	45.0	31.8	57.7	53.6	11.0	0.0	6.674841	119327	120895	1717-1719	0.962963	38.8
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	GGCAGCGTTAAAAGCTTTGT	20	20	50.0	45.0	58.0	57.9	0.0	13.5	0.142032	122296	122842	663-682	1.000000	81.4
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	GGCAGCGTTAAAAGCTTTGT	20	20	50.0	45.0	58.0	57.9	0.0	13.5	0.143402	122296	122842	664-683	1.000000	81.4
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	AAAGGATATTGGGCAGCGTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.151706	122296	122842	674-693	1.000000	81.3
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	AAAGGATATTGGGCAGCGTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.153076	122296	122842	675-694	1.000000	81.3
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TCCTAAGAGCAGCGTGTCTA	GGCAGCGTTAAAAGCTTTGT	20	20	50.0	45.0	57.9	57.9	0.0	13.5	0.258478	122296	122842	673-692	1.000000	80.4
rpl32-ndhF_p1	rpl32-ndhF	1	ACAAAGCTTTTAACGCTGCC	GTTGCCGTCGGAAAAAGAAG	20	20	45.0	50.0	57.9	58.0	9.5	17.5	0.166615	123008	124024	921-1212	1.000000	83.7
rpl32-ndhF_p2	rpl32-ndhF	2	AACGCTGCCCAATATCCTTT	GTTGCCGTCGGAAAAAGAAG	20	20	45.0	50.0	57.9	58.0	0.0	17.5	0.176290	123008	124024	910-1201	1.000000	83.7
rpl32-ndhF_p3	rpl32-ndhF	3	ACAAAGCTTTTAACGCTGCC	TGCCGTCGGAAAAAGAAGAA	20	20	45.0	45.0	57.9	58.0	9.5	17.5	0.177905	123008	124024	919-1210	1.000000	83.6
rpl32-ndhF_p4	rpl32-ndhF	4	ACAAAGCTTTTAACGCTGCC	TTGCCGTCGGAAAAAGAAGA	20	20	45.0	45.0	57.9	58.0	9.5	17.5	0.177905	123008	124024	920-1211	1.000000	83.6
rpl32-ndhF_p5	rpl32-ndhF	5	AACGCTGCCCAATATCCTTT	TGCCGTCGGAAAAAGAAGAA	20	20	45.0	45.0	57.9	58.0	0.0	17.5	0.187579	123008	124024	908-1199	1.000000	83.6