Hotspot

ycf1

SSC · 122532-127532 · 5001 bp

Back to Leontodon

Pi 0.0063

MarkerSeek score 32.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5001
variable_sites: 48
pi: 0.0063
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGTCTTCTTCTTCGCCCATT	ACATTGGATCTAAGAAGTCCC	50.0	42.9	57.9	54.7	171-204 bp	1.0	47.2
ycf1_p2	GGTCTTCTTCTTCGCCCATT	AGTACATTGGATCTAAGAAGTCCC	50.0	41.7	57.9	57.5	174-207 bp	1.0	45.7
ycf1_p3	TCAAGGTCTTCTTCTTCGCC	ACATTGGATCTAAGAAGTCCC	50.0	42.9	57.5	54.7	175-208 bp	1.0	44.5
ycf1_p4	CAAGGTCTTCTTCTTCGCCC	ACATTGGATCTAAGAAGTCCC	55.0	42.9	58.6	54.7	174-207 bp	1.0	43.7
ycf1_p5	TCAAGGTCTTCTTCTTCGCC	AGTACATTGGATCTAAGAAGTCCC	50.0	41.7	57.5	57.5	178-211 bp	1.0	42.9