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atpA

LSC · 28194-29720 · 1527 bp

Back to Leontodon

Pi 0.000764

MarkerSeek score 49.40

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 2
pi: 0.000764
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TTGGCATATTGGGGACGATG	ATCCGAAACGCTTGAGTACC	50.0	50.0	58.0	58.0	1688-1690 bp	1.0	79.9
atpA_p2	TTGGCATATTGGGGACGATG	TGAATCCGAAACGCTTGAGT	50.0	45.0	58.0	57.8	1691-1693 bp	1.0	78.6
atpA_p3	TTGGCATATTGGGGACGATG	CGAAACGCTTGAGTACCCTT	50.0	50.0	58.0	58.2	1685-1687 bp	1.0	78.3
atpA_p4	GGCATATTGGGGACGATGAA	ATCCGAAACGCTTGAGTACC	50.0	50.0	57.7	58.0	1686-1688 bp	1.0	77.9
atpA_p5	GGCATATTGGGGACGATGAA	TGAATCCGAAACGCTTGAGT	50.0	45.0	57.7	57.8	1689-1691 bp	1.0	76.6