Hotspot

accD

LSC · 56927-58366 · 1440 bp

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Pi 0.00463

MarkerSeek score 43.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1440
variable_sites: 10
pi: 0.00463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTTTCAATACTCACACTCTTTTT	TGTCAACCCCATTCGTCTAA	26.1	45.0	52.8	56.5	1609-1623 bp	1.0	41.2
accD_p2	TTTTTCAATACTCACACTCTTTT	TGTCAACCCCATTCGTCTAA	26.1	45.0	52.8	56.5	1610-1624 bp	1.0	41.2
accD_p3	TTTTTCAATACTCACACTCTTTTT	TGTCAACCCCATTCGTCTAA	25.0	45.0	53.6	56.5	1610-1624 bp	1.0	41.2
accD_p4	TTTTCAATACTCACACTCTTTTT	ATGTCAACCCCATTCGTCTA	26.1	45.0	52.8	56.2	1610-1624 bp	1.0	41.2
accD_p5	TTTTCAATACTCACACTCTTTTT	TATGTCAACCCCATTCGTCT	26.1	45.0	52.8	56.2	1611-1625 bp	1.0	41.2