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rps15-ycf1

SSC · 122133-122531 · 399 bp

Back to Leontodon

Pi 0.0117

MarkerSeek score 31.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 399
variable_sites: 7
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACTTGAAATTCAACAGATCCTCT	CGCTTGAGTATGTTAAGGAT	34.8	40.0	55.8	52.8	518-523 bp	1.0	41.1
rps15-ycf1_p2	CAGATCCTCTGTTTTCTTTGT	CGCTTGAGTATGTTAAGGAT	38.1	40.0	53.7	52.8	505-510 bp	1.0	41.1
rps15-ycf1_p3	AGTCTCCGTATCTTATTGGT	CGCTTGAGTATGTTAAGGAT	40.0	40.0	52.9	52.8	548-553 bp	1.0	41.0
rps15-ycf1_p4	AAGTCTCCGTATCTTATTGGT	CGCTTGAGTATGTTAAGGAT	38.1	40.0	53.8	52.8	549-554 bp	1.0	41.0
rps15-ycf1_p5	AGTAAGTCTCCGTATCTTATTGGT	CGCTTGAGTATGTTAAGGAT	37.5	40.0	56.7	52.8	552-557 bp	1.0	41.0