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trnG-GCC-trnfM-CAU

LSC · 35945-36147 · 203 bp

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Pi 0.00328

MarkerSeek score 50.20

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 1
pi: 0.00328
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GAACGAAACAAAACGCGGAT	GGGTAGAGCAGTTTGGTAGC	45.0	55.0	58.0	58.0	358-376 bp	1.0	83.8
trnG-GCC-trnfM-CAU_p2	GAACGAAACAAAACGCGGAT	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	322-340 bp	1.0	83.7
trnG-GCC-trnfM-CAU_p3	GAACGAAACAAAACGCGGAT	TTCAGTTCTTCTCTTCGGCG	45.0	50.0	58.0	57.9	380-398 bp	1.0	83.5
trnG-GCC-trnfM-CAU_p4	GAACGAAACAAAACGCGGAT	GTCACGGGTTCAAATCCTGT	45.0	50.0	58.0	58.1	316-334 bp	1.0	83.2
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	317-335 bp	1.0	82.7