Hotspot

rpl16

LSC · 82591-84066 · 1476 bp

Back to Leontice

Pi 0.00758

MarkerSeek score 55.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 11
pi: 0.00758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGGCCCTTTCTTTTGGTTACA	TGATTGACCTTTGGTATAATTGCT	42.9	33.3	57.7	56.7	1504-1526 bp	1.0	41.9
rpl16_p2	GGCCCTTTCTTTTGGTTACAC	TGATTGACCTTTGGTATAATTGCT	47.6	33.3	57.6	56.7	1503-1525 bp	1.0	41.9
rpl16_p3	AGGCCCTTTCTTTTGGTTACA	TTGATTGACCTTTGGTATAATTGCT	42.9	32.0	57.7	57.3	1505-1527 bp	1.0	41.9
rpl16_p4	AGGCCCTTTCTTTTGGTTACA	TGATTGACCTTTGGTATAATTGC	42.9	34.8	57.7	55.3	1504-1526 bp	1.0	41.9
rpl16_p5	GGCCCTTTCTTTTGGTTACAC	TTGATTGACCTTTGGTATAATTGCT	47.6	32.0	57.6	57.3	1504-1526 bp	1.0	41.9