Hotspot

ndhF-rpl32

SSC · 114253-115037 · 785 bp

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Pi 0.0148

MarkerSeek score 68.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 785
variable_sites: 11
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACGAAGACTTTTTGTTGCCG	AGTACGTTTCTTCGGAACCG	45.0	50.0	57.5	57.9	887-920 bp	1.0	80.3
ndhF-rpl32_p2	GAAGACTTTTTGTTGCCGCC	AGTACGTTTCTTCGGAACCG	50.0	50.0	58.5	57.9	885-918 bp	1.0	80.0
ndhF-rpl32_p3	ACGAAGACTTTTTGTTGCCG	GCTGCCCAATACCCTTTGAT	45.0	50.0	57.5	58.2	946-979 bp	1.0	78.7
ndhF-rpl32_p4	GAAGACTTTTTGTTGCCGCC	GCTGCCCAATACCCTTTGAT	50.0	50.0	58.5	58.2	944-977 bp	1.0	78.4
ndhF-rpl32_p5	CCCACTCCGATTGCTATAGG	AGTACGTTTCTTCGGAACCG	55.0	50.0	57.2	57.9	850-883 bp	1.0	78.3