Hotspot

rpl14-rpl16

LSC · 82470-82590 · 121 bp

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Pi 0.0776

MarkerSeek score 76.90

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 9
pi: 0.0776
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTGTTGTCTGCTACATT	AAATTGCAGCCAGAGAAGCT	50.0	45.0	58.6	58.1	240-242 bp	1.0	79.6
rpl14-rpl16_p2	CCGCTGTTGTCTGCTACATT	GAAATTGCAGCCAGAGAAGC	50.0	50.0	58.6	57.7	241-243 bp	1.0	78.0
rpl14-rpl16_p3	CCGCTGTTGTCTGCTACATT	AATAGCTGCGTCCAAAATGC	50.0	45.0	58.6	57.4	215-217 bp	1.0	76.2
rpl14-rpl16_p4	CCGCTGTTGTCTGCTACATT	GCGTCCAAAATGCCTATACG	50.0	50.0	58.6	57.3	208-210 bp	1.0	75.6
rpl14-rpl16_p5	CCGCTGTTGTCTGCTACAT	AAATTGCAGCCAGAGAAGCT	52.6	45.0	57.9	58.1	240-242 bp	1.0	74.9