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rpl33-rps18

LSC · 68970-69234 · 265 bp

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Pi 0.0282

MarkerSeek score 68.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 265
variable_sites: 7
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGACACAATACGCCT	ATTCGATCCCCCGATCCAAT	45.0	50.0	57.0	58.6	437-466 bp	1.0	71.5
rpl33-rps18_p2	AGAATCGACACAATACGCCT	ATCAATTCGATCCCCCGATC	45.0	50.0	57.0	57.2	441-470 bp	1.0	70.4
rpl33-rps18_p3	CGACACAATACGCCTAATCG	ATTCGATCCCCCGATCCAAT	50.0	50.0	56.8	58.6	432-461 bp	1.0	69.8
rpl33-rps18_p4	AGAATCGACACAATACGCCT	TTCGATCCCCCGATCCAATT	45.0	50.0	57.0	58.9	436-465 bp	1.0	69.7
rpl33-rps18_p5	AGAATCGACACAATACGCCT	AATTCGATCCCCCGATCCAA	45.0	50.0	57.0	58.9	438-467 bp	1.0	69.6