Hotspot

psaA-ycf3

LSC · 43077-43549 · 473 bp

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Pi 0.0217

MarkerSeek score 71.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 10
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	572-584 bp	1.0	80.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TGGTTCAATCAAGCTGCTGA	50.0	45.0	57.9	57.7	646-658 bp	1.0	79.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	613-625 bp	1.0	79.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGGTTTGAATAA	50.0	45.0	57.9	57.5	561-573 bp	1.0	78.6
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	GATCACGAGGCGGTTTGAAT	50.0	50.0	57.9	58.6	563-575 bp	1.0	77.3