Hotspot

petN-psbM

LSC · 28846-29679 · 834 bp

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Pi 0.00506

MarkerSeek score 66.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 4
pi: 0.00506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCATTGTTCATTCT	45.0	38.1	58.0	55.9	983-989 bp	1.0	58.8
petN-psbM_p2	CGTAGTATGGGGAAGAAGCG	TGCTACTGCATTGTTCATTCT	55.0	38.1	57.9	55.9	935-941 bp	1.0	58.0
petN-psbM_p3	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCATTGTTCATTCT	50.0	38.1	57.8	55.9	989-995 bp	1.0	57.3
petN-psbM_p4	GGGGGCATAATTCACATGGA	TGCTACTGCATTGTTCATTCT	50.0	38.1	57.6	55.9	1012-1018 bp	1.0	55.7
petN-psbM_p5	AGGGGGCATAATTCACATGG	TGCTACTGCATTGTTCATTCT	50.0	38.1	57.6	55.9	1013-1019 bp	1.0	55.6