Hotspot

rpl16

LSC · 85847-87560 · 1714 bp

Back to Lemna

Pi 0.0214
MarkerSeek score 61.80
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1714
variable_sites
36
pi
0.0214
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CCGTTTTCACGCTTGAGTTC TCCTCTACAAACCATTCGCG 50.0 50.0 58.0 58.0 2305-2318 bp 1.0 82.5
rpl16_p2 CCGTTTTCACGCTTGAGTTC ATCGGCGACTTAACATTGCT 50.0 45.0 58.0 58.0 2537-2550 bp 1.0 82.2
rpl16_p3 TCTCAATTCCCTCGCGATTG TCCTCTACAAACCATTCGCG 50.0 50.0 58.1 58.0 2405-2418 bp 1.0 82.1
rpl16_p4 CCGTTTTCACGCTTGAGTTC GGTTTCGAAGGAATTGCACG 50.0 50.0 58.0 58.0 2691-2704 bp 1.0 82.0
rpl16_p5 GTGGATGCTATTCGAACCGT TCCTCTACAAACCATTCGCG 50.0 50.0 58.1 58.0 2693-2717 bp 1.0 82.0