Hotspot

psaC-ndhE

SSC · 128399-129238 · 840 bp

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Pi 0.0615
MarkerSeek score 79.00
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
840
variable_sites
48
pi
0.0615
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 GCCTTACATCCATCCCAAGG GTCGAAGCATGGTTAGAGCA 55.0 50.0 58.0 58.0 1155-1183 bp 1.0 87.4
psaC-ndhE_p2 GCCTTACATCCATCCCAAGG TCGAAGCATGGTTAGAGCAC 55.0 50.0 58.0 58.0 1154-1182 bp 1.0 87.4
psaC-ndhE_p3 GCCTTACATCCATCCCAAGG CGAAGCATGGTTAGAGCACT 55.0 50.0 58.0 58.0 1153-1181 bp 1.0 87.3
psaC-ndhE_p4 AAGCCGCTAAAGTCGCTAAA TCGAAGCATGGTTAGAGCAC 45.0 50.0 57.9 58.0 1738-1773 bp 1.0 85.0
psaC-ndhE_p5 GCCTTACATCCATCCCAAGG TAGCAAGGGGTGAAGACAGA 55.0 50.0 58.0 58.0 1447 bp 0.5 59.7