Hotspot

rps15-ndhF

IRb · 120930-121243 · 314 bp

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Pi 0.0638
MarkerSeek score 72.90
Primer available yes
Label rps15-ndhF

Feature Metadata

label_name
rps15-ndhF
feature_type
igs
region
IRb
length_bp
314
variable_sites
19
pi
0.0638
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ndhF_p1 CAGTTGGATATTCGGGAGCC GTAGGAGGGGGTCGAATCTC 55.0 60.0 58.1 58.7 434-460 bp 1.0 80.3
rps15-ndhF_p2 CAGTTGGATATTCGGGAGCC AGGGGGTCGAATCTCCTTTT 55.0 50.0 58.1 58.3 429 bp 0.5 56.4
rps15-ndhF_p3 GTTGGATATTCGGGAGCCAA AGGGGGTCGAATCTCCTTTT 50.0 50.0 57.7 58.3 427 bp 0.5 54.6
rps15-ndhF_p4 GTGTAGTGTGTAGTGCGTGA AGGGGGTCGAATCTCCTTTT 50.0 50.0 57.6 58.3 1047 bp 0.5 54.1
rps15-ndhF_p5 AGTGTAGTGTGTAGTGCGTG AGGGGGTCGAATCTCCTTTT 50.0 50.0 57.6 58.3 1048 bp 0.5 54.0