Hotspot

rps19-rpl2

LSC · 89470-89521 · 52 bp

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Pi 0.0455
MarkerSeek score 68.40
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
LSC
length_bp
52
variable_sites
2
pi
0.0455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 ACCAATAAATGATTAGCTACAAAAGGA CGTTTTATTCTTCGTCGCCG 29.6 50.0 57.5 57.9 121-129 bp 1.0 45.3
rps19-rpl2_p2 ACCAATAAATGATTAGCTACAAAAGG CGTTTTATTCTTCGTCGCCG 30.8 50.0 56.3 57.9 121-129 bp 1.0 45.3
rps19-rpl2_p3 ACCAATAAATGATTAGCTACAAAAGGA TTGGGGTTATCCTGCACTTG 29.6 50.0 57.5 57.8 176-184 bp 1.0 44.4
rps19-rpl2_p4 ACCAATAAATGATTAGCTACAAAAGG TTGGGGTTATCCTGCACTTG 30.8 50.0 56.3 57.8 176-184 bp 1.0 44.4
rps19-rpl2_p5 ACCAATAAATGATTAGCTACAAAAGGA AAAAGACCCATGACCCCTTG 29.6 50.0 57.5 57.7 193-201 bp 1.0 44.1