Hotspot

rpl36-rps8

LSC · 84577-84804 · 228 bp

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Pi 0.0395
MarkerSeek score 71.40
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
228
variable_sites
9
pi
0.0395
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 TATAATTCGTCCGCGCCTAC AGCTCGCCTAAAAGGAGTTG 50.0 50.0 57.7 57.9 349-353 bp 1.0 78.6
rpl36-rps8_p2 TACTATAATTCGTCCGCGCC TAGGATTTTGGGCGGAATGG 50.0 50.0 57.7 57.9 418-422 bp 1.0 78.5
rpl36-rps8_p3 TATAATTCGTCCGCGCCTAC TAGGATTTTGGGCGGAATGG 50.0 50.0 57.7 57.9 415-419 bp 1.0 78.5
rpl36-rps8_p4 TACTATAATTCGTCCGCGCC AGCTCGCCTAAAAGGAGTTG 50.0 50.0 57.7 57.9 352-356 bp 1.0 78.5
rpl36-rps8_p5 TATAATTCGTCCGCGCCTAC ATTTTGGGCGGAATGGGAAT 50.0 45.0 57.7 58.1 411-415 bp 1.0 78.1