Hotspot

psbE-petL

LSC · 69746-70871 · 1126 bp

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Pi 0.0194
MarkerSeek score 68.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1126
variable_sites
21
pi
0.0194
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ACTGACGAATAACCAACCCG CCAATGAAAAGCACTGAGGC 50.0 50.0 57.9 57.6 1274-1314 bp 1.0 80.4
psbE-petL_p2 ACGAATAACCAACCCGCAAT CCAATGAAAAGCACTGAGGC 45.0 50.0 58.2 57.6 1270-1310 bp 1.0 79.7
psbE-petL_p3 ACTGACGAATAACCAACCCG AGCAGCCAATAGAAAACCGA 50.0 45.0 57.9 57.5 1245-1285 bp 1.0 79.4
psbE-petL_p4 ACGAATAACCAACCCGCAAT AGCAGCCAATAGAAAACCGA 45.0 45.0 58.2 57.5 1241-1281 bp 1.0 78.8
psbE-petL_p5 ACAAAAGAACGTTCTCCCGT CCAATGAAAAGCACTGAGGC 45.0 50.0 57.7 57.6 1189-1229 bp 1.0 78.8