Hotspot

tRNA-Leu(UAA)-tRNA-Phe(GAA)

LSC · 51919-52052 · 134 bp

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Pi 0.0448
MarkerSeek score 69.70
Primer available yes
Label tRNA-Leu(UAA)-tRNA-Phe(GAA)

Feature Metadata

label_name
tRNA-Leu(UAA)-tRNA-Phe(GAA)
feature_type
igs
region
LSC
length_bp
134
variable_sites
6
pi
0.0448
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Leu(UAA)-tRNA-Phe(GAA)_p1 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 224-237 bp 1.0 77.9
tRNA-Leu(UAA)-tRNA-Phe(GAA)_p2 AATCGTGAGGGTTCAAGTCC TACCAACTGAGCTATCCCGA 50.0 50.0 57.5 57.6 186-199 bp 1.0 75.1
tRNA-Leu(UAA)-tRNA-Phe(GAA)_p3 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 50.0 50.0 57.5 57.5 221-234 bp 1.0 74.7
tRNA-Leu(UAA)-tRNA-Phe(GAA)_p4 TTCAAGTCCCTCTATCCCCA TTTGAACTGGTGACACGAGG 50.0 50.0 57.0 58.1 213-226 bp 1.0 74.1
tRNA-Leu(UAA)-tRNA-Phe(GAA)_p5 AATCGTGAGGGTTCAAGTCC TCCTCTGCTCTACCAACTGA 50.0 50.0 57.5 57.4 196-209 bp 1.0 73.7