Hotspot

psbC-tRNA-Ser(UGA)

LSC · 38514-38625 · 112 bp

Back to Lemna

Pi 0.0357
MarkerSeek score 68.80
Primer available yes
Label psbC-tRNA-Ser(UGA)

Feature Metadata

label_name
psbC-tRNA-Ser(UGA)
feature_type
igs
region
LSC
length_bp
112
variable_sites
4
pi
0.0357
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-tRNA-Ser(UGA)_p1 TTGTGGGTCATTTGTGGCAT GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.3 58.1 295 bp 1.0 78.3
psbC-tRNA-Ser(UGA)_p2 AGCTGCAGCAGGATTTGAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.4 58.1 258 bp 1.0 77.8
psbC-tRNA-Ser(UGA)_p3 TTGTGGGTCATTTGTGGCAT GATGGCCGAGTGGTTGATAG 45.0 55.0 58.3 58.1 306 bp 1.0 77.7
psbC-tRNA-Ser(UGA)_p4 TTGTGGGTCATTTGTGGCAT GGGTTCGAATCCCTCTCTCT 45.0 55.0 58.3 58.2 245 bp 1.0 77.3
psbC-tRNA-Ser(UGA)_p5 GCTGCAGCAGGATTTGAAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 57.6 58.1 257 bp 1.0 77.2