Hotspot

tRNA-Thr(GGU)-psbD

LSC · 34943-36082 · 1140 bp

Back to Lemna

Pi 0.0337
MarkerSeek score 70.30
Primer available yes
Label tRNA-Thr(GGU)-psbD

Feature Metadata

label_name
tRNA-Thr(GGU)-psbD
feature_type
igs
region
LSC
length_bp
1140
variable_sites
38
pi
0.0337
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Thr(GGU)-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTACGTAACC 55.0 55.0 58.1 58.0 1282-1288 bp 1.0 82.6
tRNA-Thr(GGU)-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1285-1291 bp 1.0 82.4
tRNA-Thr(GGU)-psbD_p3 GTGGTAGAGTAACGCCATGG CACAAAAACGAAACGGTCCC 55.0 50.0 58.1 58.2 1291-1297 bp 1.0 81.3
tRNA-Thr(GGU)-psbD_p4 GTGGTAGAGTAACGCCATGG CCACAAAAACGAAACGGTCC 55.0 50.0 58.1 58.2 1292-1298 bp 1.0 81.3
tRNA-Thr(GGU)-psbD_p5 GTGGTAGAGTAACGCCATGG CCCACAAAAACGAAACGGTC 55.0 50.0 58.1 58.2 1293-1299 bp 1.0 81.3