Hotspot

tRNA-Cys(GCA)-petN

LSC · 29765-30806 · 1042 bp

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Pi 0.0351
MarkerSeek score 76.10
Primer available yes
Label tRNA-Cys(GCA)-petN

Feature Metadata

label_name
tRNA-Cys(GCA)-petN
feature_type
igs
region
LSC
length_bp
1042
variable_sites
35
pi
0.0351
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Cys(GCA)-petN_p1 GGTGTCGCCTGATCAAGAAA AGCCCAAGCGAGACTTACTA 50.0 50.0 58.2 58.2 1048-1089 bp 1.0 83.3
tRNA-Cys(GCA)-petN_p2 GGTGTCGCCTGATCAAGAAA CCACTCCTACCCCAGACTAC 50.0 60.0 58.2 58.2 1104-1145 bp 1.0 82.5
tRNA-Cys(GCA)-petN_p3 GGTGTCGCCTGATCAAGAAA TCCACTCCTACCCCAGACTA 50.0 55.0 58.2 58.3 1105-1146 bp 1.0 81.7
tRNA-Cys(GCA)-petN_p4 GGTGTCGCCTGATCAAGAAA AAAGTCCACTCCTACCCCAG 50.0 55.0 58.2 58.3 1109-1150 bp 1.0 81.5
tRNA-Cys(GCA)-petN_p5 GGTGTCGCCTGATCAAGAAA CCGTACCCCTAAAGTCCACT 50.0 55.0 58.2 58.4 1160 bp 0.5 55.0