Hotspot

rpoB-tRNA-Cys(GCA)

LSC · 28235-29683 · 1449 bp

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Pi 0.0247
MarkerSeek score 69.10
Primer available yes
Label rpoB-tRNA-Cys(GCA)

Feature Metadata

label_name
rpoB-tRNA-Cys(GCA)
feature_type
igs
region
LSC
length_bp
1449
variable_sites
34
pi
0.0247
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-tRNA-Cys(GCA)_p1 ATTCCCTCATTTCCATCGCG TTTCTTGATCAGGCGACACC 50.0 50.0 58.4 58.2 1514-1556 bp 1.0 80.7
rpoB-tRNA-Cys(GCA)_p2 ATTCCCTCATTTCCATCGCG GCGACACCCAGATTTGAACT 50.0 50.0 58.4 58.5 1502-1544 bp 1.0 78.5
rpoB-tRNA-Cys(GCA)_p3 ATTCCCTCATTTCCATCGCG TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.4 57.5 1477-1519 bp 1.0 78.2
rpoB-tRNA-Cys(GCA)_p4 ATTCCCTCATTTCCATCGCG CCTTACCACTTGGCCATGTC 50.0 55.0 58.4 58.5 1456-1498 bp 1.0 78.2
rpoB-tRNA-Cys(GCA)_p5 CATTCCCTCATTTCCATCGC TTTCTTGATCAGGCGACACC 50.0 50.0 56.9 58.2 1515-1557 bp 1.0 75.3