Hotspot

tRNA-Arg(UCU)-atpA

LSC · 11340-11517 · 178 bp

Back to Lemna

Pi 0.0417
MarkerSeek score 73.50
Primer available yes
Label tRNA-Arg(UCU)-atpA

Feature Metadata

label_name
tRNA-Arg(UCU)-atpA
feature_type
igs
region
LSC
length_bp
178
variable_sites
7
pi
0.0417
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Arg(UCU)-atpA_p1 AAAAGCAGCGTCCATTGTCT TTCTACAACAGGAGCAAGCC 45.0 50.0 58.4 57.8 272-280 bp 1.0 80.3
tRNA-Arg(UCU)-atpA_p2 AAAGCAGCGTCCATTGTCTA TTCTACAACAGGAGCAAGCC 45.0 50.0 57.5 57.8 271-279 bp 1.0 79.7
tRNA-Arg(UCU)-atpA_p3 AAGCAGCGTCCATTGTCTAA TTCTACAACAGGAGCAAGCC 45.0 50.0 57.5 57.8 270-278 bp 1.0 79.7
tRNA-Arg(UCU)-atpA_p4 AAAAGCAGCGTCCATTGTCT TTCCACCAAGACATTCACCC 45.0 50.0 58.4 57.7 343-351 bp 1.0 78.5
tRNA-Arg(UCU)-atpA_p5 AAGCAGCGTCCATTGTCTAA TTCCACCAAGACATTCACCC 45.0 50.0 57.5 57.7 341-349 bp 1.0 77.9