Hotspot

tRNA-Ser(GCU)-tRNA-Gly(UCC)

LSC · 8700-10313 · 1614 bp

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Pi 0.0435
MarkerSeek score 79.90
Primer available yes
Label tRNA-Ser(GCU)-tRNA-Gly(UCC)

Feature Metadata

label_name
tRNA-Ser(GCU)-tRNA-Gly(UCC)
feature_type
igs
region
LSC
length_bp
1614
variable_sites
67
pi
0.0435
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p1 ATTAGCAATCCGCCGCTTTA AGGACGAATCACACTTTTACCA 45.0 40.9 58.0 57.7 1691-1717 bp 1.0 67.5
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p2 ATTAGCAATCCGCCGCTTTA GGACGAATCACACTTTTACCAC 45.0 45.5 58.0 57.6 1690-1716 bp 1.0 67.1
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p3 CGGAGAGAGAGGGATTCGAA AGGACGAATCACACTTTTACCA 55.0 40.9 58.3 57.7 1739-1765 bp 1.0 65.1
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p4 CGGAGAGAGAGGGATTCGAA GGACGAATCACACTTTTACCAC 55.0 45.5 58.3 57.6 1738-1764 bp 1.0 64.6
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p5 GCTTTAGTCCACTCAGCCAT AGGACGAATCACACTTTTACCA 50.0 40.9 57.6 57.7 1677-1703 bp 1.0 64.6