Hotspot

rps16-tRNA-Gln(UUG)

LSC · 6223-7202 · 980 bp

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Pi 0.0355
MarkerSeek score 71.30
Primer available yes
Label rps16-tRNA-Gln(UUG)

Feature Metadata

label_name
rps16-tRNA-Gln(UUG)
feature_type
igs
region
LSC
length_bp
980
variable_sites
34
pi
0.0355
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-tRNA-Gln(UUG)_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1053-1078 bp 1.0 82.6
rps16-tRNA-Gln(UUG)_p2 GGATCACGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1067-1092 bp 1.0 80.7
rps16-tRNA-Gln(UUG)_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1057-1082 bp 1.0 77.8
rps16-tRNA-Gln(UUG)_p4 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1083-1108 bp 1.0 77.6
rps16-tRNA-Gln(UUG)_p5 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1045-1070 bp 1.0 77.4