Hotspot

ycf1

SSC · 122433-127535 · 5103 bp

Back to Lemmaphyllum

Pi 0.000784

MarkerSeek score 53.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5103
variable_sites: 4
pi: 0.000784
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATGGATGCCGCAAAATTGG	ACCAAATTCACCAGACTCGG	45.0	50.0	58.0	57.8	217 bp	1.0	78.7
ycf1_p2	AATGGATGCCGCAAAATTGG	CTCGGGGTTGAACGTAGATC	45.0	55.0	58.0	57.8	202 bp	1.0	78.5
ycf1_p3	GTGGCAAGTTCGCTACAGTA	ACCAAATTCACCAGACTCGG	50.0	50.0	57.9	57.8	270 bp	1.0	78.2
ycf1_p4	GTGGCAAGTTCGCTACAGTA	CTCGGGGTTGAACGTAGATC	50.0	55.0	57.9	57.8	255 bp	1.0	78.1
ycf1_p5	AATGGATGCCGCAAAATTGG	CGGGGTTGAACGTAGATCAA	45.0	50.0	58.0	57.6	200 bp	1.0	77.2