Hotspot

rbcL

LSC · 51394-52821 · 1428 bp

Back to Lemmaphyllum

Pi 0.0014

MarkerSeek score 59.60

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 2
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CACTTTGCGTTTCACGTTGA	AGTATGGGGGAGGTGAACTC	45.0	55.0	57.8	58.1	1625 bp	1.0	77.8
rbcL_p2	CACTTTGCGTTTCACGTTGA	GAGTATGGGGGAGGTGAACT	45.0	55.0	57.8	58.1	1626 bp	1.0	77.8
rbcL_p3	CACTTTGCGTTTCACGTTGA	TGTCTTACAGCTGTTGCGAA	45.0	45.0	57.8	57.8	1586 bp	1.0	76.9
rbcL_p4	TACCTATGCCGAGCAGATCT	AGTATGGGGGAGGTGAACTC	50.0	55.0	57.7	58.1	1598 bp	1.0	76.8
rbcL_p5	TACCTATGCCGAGCAGATCT	GAGTATGGGGGAGGTGAACT	50.0	55.0	57.7	58.1	1599 bp	1.0	76.8