Hotspot

petB

LSC · 69734-71196 · 1463 bp

Back to Lemmaphyllum

Pi 0.00137

MarkerSeek score 59.70

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1463
variable_sites: 2
pi: 0.00137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CCGCTGACGTAACTAATGGT	CTTCTCCCACCCCTGACTTA	50.0	55.0	57.7	58.0	1607-1609 bp	1.0	77.5
petB_p2	CCGCTGACGTAACTAATGGT	ATCATCGGCGACAGAATGAC	50.0	50.0	57.7	58.1	1679-1681 bp	1.0	76.7
petB_p3	CCCCGCTGACGTAACTAATG	CTTCTCCCACCCCTGACTTA	55.0	55.0	58.4	58.0	1609-1611 bp	1.0	76.4
petB_p4	CCCGCTGACGTAACTAATGG	CTTCTCCCACCCCTGACTTA	55.0	55.0	58.4	58.0	1608-1610 bp	1.0	76.4
petB_p5	CCCGCTGACGTAACTAATGG	ATCATCGGCGACAGAATGAC	55.0	50.0	58.4	58.1	1680-1682 bp	1.0	75.6