Hotspot

cemA

LSC · 55899-57398 · 1500 bp

Back to Lemmaphyllum

Pi 0.00133

MarkerSeek score 47.20

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 1500
variable_sites: 2
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TCCCAGGAAGAAATAGCCCA	TCTCTTTAGATTTGGAAAGGTGGT	50.0	37.5	58.0	57.8	1617 bp	1.0	46.3
cemA_p2	TCTCGTATTTCGCCTGCTTC	TCTCTTTAGATTTGGAAAGGTGGT	50.0	37.5	58.1	57.8	1579 bp	1.0	45.9
cemA_p3	ATATTTCAGGGGGAGAGGGG	TCTCTTTAGATTTGGAAAGGTGGT	55.0	37.5	57.9	57.8	1637 bp	1.0	45.4
cemA_p4	GTATTTCGCCTGCTTCCTCT	TCTCTTTAGATTTGGAAAGGTGGT	50.0	37.5	57.7	57.8	1575 bp	1.0	44.0
cemA_p5	GTCCCAGGAAGAAATAGCCC	TCTCTTTAGATTTGGAAAGGTGGT	55.0	37.5	57.6	57.8	1618 bp	1.0	43.7