Hotspot

atpF

LSC · 10429-11712 · 1284 bp

Back to Lemmaphyllum

Pi 0

MarkerSeek score 59.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1284
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TGCTGCTAATTTCGTCAGGT	GCAAAACTTTCCAGGAGGGA	45.0	50.0	57.5	58.0	1427-1431 bp	1.0	76.4
atpF_p2	TGCTGCTAATTTCGTCAGGT	CGGACCAGAGGTTGTTCAAA	45.0	50.0	57.5	58.0	1451-1455 bp	1.0	76.1
atpF_p3	CCCGCGTGATGAGATGAAAA	GCAAAACTTTCCAGGAGGGA	50.0	50.0	58.6	58.0	1345-1349 bp	1.0	75.1
atpF_p4	CCCGCGTGATGAGATGAAAA	CGGACCAGAGGTTGTTCAAA	50.0	50.0	58.6	58.0	1369-1373 bp	1.0	74.9
atpF_p5	TAAAACCCGCGTGATGAGAT	GCAAAACTTTCCAGGAGGGA	45.0	50.0	57.3	58.0	1350-1354 bp	1.0	74.7