Hotspot

trnR-ACG-trnT-UGU

IRa · 153713-154448 · 736 bp

Back to Lemmaphyllum

Pi 0.00136

MarkerSeek score 59.50

Primer available yes

Label trnR-ACG-trnT-UGU

Feature Metadata

label_name: trnR-ACG-trnT-UGU
feature_type: igs
region: IRa
length_bp: 736
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnT-UGU_p1	TGAAACGGATCCAGTAGGGT	TGCCTCAAAAGAGGGGTAGA	50.0	50.0	58.1	58.0	913 bp	1.0	79.4
trnR-ACG-trnT-UGU_p2	TGAAACGGATCCAGTAGGGT	AGTACGGAGGTTCGAATCCT	50.0	50.0	58.1	57.9	864 bp	1.0	78.5
trnR-ACG-trnT-UGU_p3	TGAAACGGATCCAGTAGGGT	TTTCCCTGCCTCAAAAGAGG	50.0	50.0	58.1	57.7	919 bp	1.0	77.3
trnR-ACG-trnT-UGU_p4	TGAAACGGATCCAGTAGGGT	GGATTGGTGAGCGTAACTCC	50.0	55.0	58.1	58.3	945 bp	1.0	76.9
trnR-ACG-trnT-UGU_p5	TGAAACGGATCCAGTAGGGT	CGTTTCCCTGCCTCAAAAGA	50.0	50.0	58.1	58.4	921 bp	1.0	76.5