Hotspot

petA-psbJ

LSC · 58578-59263 · 686 bp

Back to Lemmaphyllum

Pi 0.00146

MarkerSeek score 59.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 1
pi: 0.00146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTCAGTTGTCTTGGCACAGA	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	848-849 bp	1.0	79.3
petA-psbJ_p2	TTGTGTCAGTTGTCTTGGCA	GCCGGTACACTTGTGATAGG	45.0	55.0	57.9	58.1	852-853 bp	1.0	78.7
petA-psbJ_p3	TTGGCACAGATTTTCCTCGT	GCCGGTACACTTGTGATAGG	45.0	55.0	57.7	58.1	838-839 bp	1.0	77.5
petA-psbJ_p4	GTCAGTTGTCTTGGCACAGA	CTGTAGCCGGTACACTTGTG	50.0	55.0	58.0	58.3	853-854 bp	1.0	77.5
petA-psbJ_p5	GTCAGTTGTCTTGGCACAGA	AGGATTCCCCTATGGTTGGT	50.0	50.0	58.0	57.7	878-879 bp	1.0	77.4