Hotspot

rps4-trnL-UAA

LSC · 43652-44283 · 632 bp

Back to Lemmaphyllum

Pi 0.00158

MarkerSeek score 59.70

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 1
pi: 0.00158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	TCGGTAGCGACTCGAAATTC	ATTGCCCGATAAATGGTCCC	50.0	50.0	57.8	58.0	861 bp	1.0	78.6
rps4-trnL-UAA_p2	CGGTAGCGACTCGAAATTCT	ATTGCCCGATAAATGGTCCC	50.0	50.0	57.8	58.0	860 bp	1.0	78.5
rps4-trnL-UAA_p3	TCGGTAGCGACTCGAAATTC	CTCCACATCCAGGCTCAATC	50.0	55.0	57.8	58.0	804 bp	1.0	78.3
rps4-trnL-UAA_p4	CGGTAGCGACTCGAAATTCT	CTCCACATCCAGGCTCAATC	50.0	55.0	57.8	58.0	803 bp	1.0	78.3
rps4-trnL-UAA_p5	GCGACTCGAAATTCTCCCAA	ATTGCCCGATAAATGGTCCC	50.0	50.0	58.3	58.0	855 bp	1.0	77.8