Hotspot

psbD-trnT-GGU

LSC · 32810-34227 · 1418 bp

Back to Lemmaphyllum

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1418
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CGAAGACGAAACGGTCTCTT	GTAGTTGTGACTTGAGGCCC	50.0	55.0	58.0	58.2	1597 bp	1.0	78.2
psbD-trnT-GGU_p2	ACGGTCTCTTCTTAGCCAGT	GTAGTTGTGACTTGAGGCCC	50.0	55.0	58.1	58.2	1587 bp	1.0	78.0
psbD-trnT-GGU_p3	TCTTCTTAGCCAGTCGTCCA	GTAGTTGTGACTTGAGGCCC	50.0	55.0	58.1	58.2	1581 bp	1.0	77.9
psbD-trnT-GGU_p4	TTTTCCGATGGCAATGGTCA	GTAGTTGTGACTTGAGGCCC	45.0	55.0	58.1	58.2	1527 bp	1.0	77.9
psbD-trnT-GGU_p5	TTTCCGATGGCAATGGTCAT	GTAGTTGTGACTTGAGGCCC	45.0	55.0	57.9	58.2	1526 bp	1.0	77.4