Hotspot

trnD-GUC-trnY-GUA

LSC · 26058-26109 · 52 bp

Back to Lemmaphyllum

Pi 0.0192

MarkerSeek score 63.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 52
variable_sites: 1
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	AGTTCAATCGGTTAGAGCACC	CCCTGGGGGTATTCATCTCT	47.6	55.0	58.0	57.9	227 bp	1.0	71.1
trnD-GUC-trnY-GUA_p2	GGTTCGAGACCCGTCAATC	CCCTGGGGGTATTCATCTCT	57.9	55.0	58.0	57.9	183 bp	1.0	71.1
trnD-GUC-trnY-GUA_p3	GTTCGAGACCCGTCAATCC	CCCTGGGGGTATTCATCTCT	57.9	55.0	58.0	57.9	182 bp	1.0	71.1
trnD-GUC-trnY-GUA_p4	AGTTCAATCGGTTAGAGCACC	TCTCAATTCTGGGTCGATGC	47.6	50.0	58.0	57.7	210 bp	1.0	69.6
trnD-GUC-trnY-GUA_p5	GTTCGAGACCCGTCAATCC	TCTCAATTCTGGGTCGATGC	57.9	50.0	58.0	57.7	165 bp	1.0	69.6